Figure 5. MiR-204 inhibits blood vessels formation in vivo.
(A) Directed in vivo angiogenesis assay. (B) Representative images of angioreactors extirpated from nude mice 9 days after implantation. Inductor of angiogenesis VEGF and FGF1 or MDA-MB-231 cells transfected with miR-204 or scramble control were placed into angioreactors as described in Methods section. (C) Fluorometric quantification of blood vessels formation using FITC-lectin detection assay. Experiments were performed by duplicate tree times.