Figure 1. Particle Characterization.
(A) SEM images of uncoated particles (NPS) and particles coated with cellular membrane derived from murine macrophages (J774 LLV) and human T-cells (Jurkat LLV). (B) Fluorescent microscope images of LLV-modified with Alexa Fluor 555 (red, first column) and immunofluorescent staining of Jurkat LLV and J774 LLV for surface markers LFA-1 and Mac-1 (green, second column) and merged (third column). (C) Western blot analysis of leukocyte adhesion molecules successfully transferred on LLV (WCL: whole cell lysate). (D) Flow cytometry analysis of particles revealing the presence of LFA-1 and Mac-1. (E) Flow cytometry analysis of the particles stained for wheat germ agglutinin. The data are plotted as the mean ± s.d.