Figure 5. Intravital microscopy analysis of LLV tumor endothelium targeting and binding stability.

(A) Intravital microscopy images of orthotopic 4T1 tumor following treatment with NPS and LLV (scale bar: 100 μm). (B) Quantification of particles bound to tumor vasculature, count was performed on same area fraction. (C) Intravital microscope images portraying binding stability of LLV and NPS on tumor endothelium at 1 and 2 h created by merging together consecutive frames obtained from 20 sec movies (scale bar: 50 μm). Arrows indicate new (red), stable (yellow), or detached (white) particles. (D) Quantification of binding stability determined from intravital microscope images. (E) Particle motion analysis in tumor vasculature. Plotting X or Y particles position as a function of time, firmly bound particle events appear as straight lines while moving particle events appear as askew lines. (F) In the Graph we report the registered velocity of moving NPS particles compared to LLV particles which appears all in steady state. The data are plotted as the mean ± s.d.