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. 2016 Oct 5;6:34790. doi: 10.1038/srep34790

Figure 6. Gallic acid inhibits activated JNK2, which is involved in hypertrophy and fibrosis.

Figure 6

Gallic acid administration was started 2 weeks before infusion of ISP (3 days) in mice; sham + vehicle, ISP + vehicle, and ISP + gallic acid group. (A,C) Representative images of immunoblots of the hearts of ISP mice. Proteins were subjected to western blotting with anti-p-ERK1/2, ERK1/2, p-JNK1/2, and JNK1/2. GAPDH was used as a loading control. (B,D) p-ERK and p-JNK proteins were quantified by densitometry. **P < 0.01 and ***P < 0.001 versus sham + vehicle; ###P < 0.001versus ISP + vehicle. (E) Rat neonatal cardiac fibroblasts and (G) H9c2 cells were transfected with pCMV-SPORT6-JNK2 or empty vector. Western blot results for JNK1/2, collagen type I, Smad3, and GATA4. GAPDH was used as a loading control. (F,H) Proteins were quantified by densitometry. Results are shown as means ± SD of 3 independent experiments. *P < 0.05. **P < 0.01, and ***P < 0.001 versus vector-transfected cells.