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. 2016 Oct 5;6:34796. doi: 10.1038/srep34796

Figure 1. Generation of a stable isogenic radiation resistant prostate cancer cell line.

Figure 1

(A) Surviving fraction, after 60Gy cumulative dose of X-radiation, of the radiation resistant (RR-22RV1) isogenic cell line model compared with 22RV1 wild type (WT-22RV1) control and 22RV1 age matched control (AMC-22RV1). The isogenic cell line shows consistent resistance to radiation after one month of growth with no treatment (RR-22RV1 + 1M). Survival of irradiated cells was measured by clonogenic cell survival assay after X-radiation with 0–10 Gy. Points are measured as means ± SEM of at least five individual experiments. (B) Surviving fraction of aerobic and hypoxic (0.5% O2, 24 hrs) WT-22Rv1 and RR-22Rv1 cells. (C) Clonogenic survival of 22RV1-WT, 2RRv1-AMC and 22RV1-RR cells treated with 0.01, 0.5 and 0.1 nM Docetaxel for 48 hours. Points are measured as means ± SEM of at least five individual experiments. Mean ± SEM, *refers to significant differences in the RR cell line vs WT control. †refers to significant differences between RR and AMC cells. Δ refers to significant differences between AMC and WT control cells. *P ≤ 0.05, **P ≤ 0.01.