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. 2016 Sep 27;39(9):699–704. doi: 10.14348/molcells.2016.0149

Fig. 3.

Fig. 3.

Mitosis-promoting factors are decreased in sh-DRG2 cells. (A) The relative levels of cyclin B1, phospho Histone H3 (pHH3), and Cdk1 were significantly reduced in sh-DRG2 cells. Data are the means ± S.E. of three independent experiments (Filled bar, control; slashed bar, sh-DRG2) (*p < 0.05, **p <0.01). (B) Cyclin B1 and Cdk1 were detected by immunofluorescence using a confocal microscope. Fluorescence images show markedly reduced expression of cyclin B1, Cdk1 and p-Cdk1(Tyr15) in sh-DRG2 cells compared with controls (Scale bar; 10 μm).