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. 2016 Oct 5;6:34597. doi: 10.1038/srep34597

Figure 1. Rhein up-regulation of Klotho correlates with its anti-renal fibrosis function.

Figure 1

(A) Representative H&E- and Masson’s trichrome-stained kidney sections from Sham, UUO, and Rhein-treated UUO mice, administered 3 days after or 1 day before UUO, respectively (n = 6) (B) Quantification of mouse kidney fibrosis from (A). (C) Rhein increases mouse kidney Klotho expression. The kidneys from Sham and Rhein-treated mice (7 days, 3 randomly selected samples from each group) were assayed for Klotho protein by Western blot. (D) Quantification of (C). (E) Renal expressions of E-cadherin, α-SMA, and Klotho from Sham, UUO, or Rhein-treated UUO mice were assayed by Western blot (3 samples in each group). (F) Quantifications of (E). (G) Representative renal immuno-histochemistry staining of Klotho and α-SMA from Sham, UUO, and Rhein-treated UUO mice. *p < 0.05,**p < 0.01 versus Sham; #p < 0.05 versus UUO mice.