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. 2016 Oct 5;6:34597. doi: 10.1038/srep34597

Figure 6. Klotho is essential for Rhein protection against renal fibrosis.

Figure 6

Representative H&E- and Masson’s trichrome-stained kidney sections from siRNA-scrambled- or siRNA-Klotho-injected Sham, UUO, or Rhein-treated UUO mice (administered 1 day before UUO) (B) Quantification of mouse kidney fibrosis (calculated as the percentage of blue-stained area over the total area) from (A). (C) Western blot examination of renal Klotho, E-cadherin (E-cad), α-SMA, β-catenin (β-cat), phosphorylated and total Smad3 from siRNA-scrambled or siRNA-Klotho treated Sham, UUO, or Rhein/UUO mice. (n = 6, 2 randomly selected samples from each group were shown). (D) Quantifications of (C). *p < 0.05,**p < 0.01 versus Sham or #p < 0.05, ##p < 0.01 versus UUO in siRNA-scrambled kidneys; △p < 0.05, △△p < 0.01 versus Sham in siRNA-Klotho-injected kidneys.