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. 2016 Oct 4;16:231. doi: 10.1186/s12866-016-0850-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — EMSAs of cdPadR1 binding the cdpadR1 promoter (P_cdpadR1), a norV promoter (P_norV), and b obg promoter (P_obg). The predicted -10 and -35 sites are indicated in blue boxes above the sequence. Orange arrows indicate the inverted repeats TACT/AGTA. c-e Final P_cdpadR1, P_norV, and P_obg (100 bp) concentration in the reaction was 0.1 μM. cdPadR1 was 5, 10, 20, and 40-fold excess over dsDNA. The minus (-) lane contains dsDNA and no cdPadR1. Shifted DNA-protein complexes are annotated with a black arrow and unbound dsDNA migration is marked with a red arrow. f For this EMSA, the final heparin concentration was 4-fold of the standard EMSA concentration used throughout this research. The + lane contains 40-fold excess cdPadR1 over dsDNA