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. 2016 Aug 10;20:254. doi: 10.1186/s13054-016-1417-z

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunofluorescent staining of sham-operated (SHAM) rat and ICU rat muscle samples. Longitudinal sections of rat tibialis anterior (TA) muscle stained with antibodies to Na⁺ channels (a, b) or dihydropyridine receptor (DHPR) (c, d) in SHAM rat and ICU rat muscles. The discrete and regular white punctate staining in SHAM muscle fibers is less obvious in ICU rat muscle fibers. e-f Cross-sections of SHAM rat (e, g) and ICU rat (f, h) muscles stained for ryanodine receptor (RyR) (e, f) or sarco-endoplasmic reticulum Ca²⁺ ATPase 1 (SERCA1) (g, h) proteins. Majority of SHAM rat muscle fibers show gray or bright staining closer to the sarcolemma. In contrast, staining is less bright and less extensive in ICU rat muscle fibers. Images are representative of three TA muscles from SHAM and ICU rats, respectively. White asterisks (b, d, f, h) indicate fibers that were considered examples of positive staining. Scale bar, bottom right (a-d) represents 20 μm and 40 μm (e-h)