Table 3.
Proteins separated by 2-DE electrophoresis and identified by MS in muscle tissue
| Spota | Identification (Swiss-Prot)b | Genec | Sequencec coverage (%) | Spotd MW (KDa) | Spote pI | Scoref | Swissg Prot access # |
|---|---|---|---|---|---|---|---|
| 1 | Phosphoglucomutase-1 (PGM1) | Pgm1 | 45 | 61.7 | 6.1 | 816 | Q9DOF7 |
| 2 | Fatty acid-binding protein, heart (FABPH) | Fabp3 | 59 | 14.8 | 6.1 | 790 | P11404 |
| 3 | Fatty acid-binding protein, heart (FABPH) | Fabp3 | 66 | 14.8 | 6.1 | 533 | P11404 |
| 4 | Galectin-1 (LEG1) | Lgals1 | 31 | 15.2 | 5.3 | 135 | P16045 |
| 5 | Fumarate hydratase, mitochondrial (FUMH) | Fh | 1 | 54.5 | 9.1 | 15 | P97807 |
a2-DE gel image spot number presented in Fig. 1
bCommonly used protein name
cGene symbol
dPercentage of amino acid sequences for the identified protein
eMW and pI = molecular weight and isoelectric point of the protein
fScore = [−10 log (P)] and P is the absolute probability that the observed match between the experimental data and the database sequence is a random event, obtained with MASCOT for all proteins listed
gSwiss-Prot primary protein accession number