Fig. 3.

Assessment of DoG particle picking with different scaling factors. a Precision and recall curves for DoG particle picking for different scaling factors (colored curves), applied on tomograms at crowding level 1 (2000 particles) with SNR = 50. Each point defines the recall and precision using a different density threshold t. Optimal F-score performance is shown by a diamond. b Boxplot of F-score distributions for particle picking at different threshold levels t for each DoG scaling factor. The diamond shaped points show the best F-score for each scaling factor. c The impact of particle size on the DoG performance with different scaling factors. The pie charts indicate the proportions of correctly predicted particle locations for complexes of different sizes (small, medium, and large complexes) for particle picking with different DoG scaling factors