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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Mol Biol. 2016;1354:367–376. doi: 10.1007/978-1-4939-3046-3_25

Fig. 2.

Fig. 2

HIV-1ADA-infected supernatant causes neuronal apoptosis in primary human neurons. HIV-1ADA-infected macrophage supernatant and uninfected macrophage supernatant were diluted with 100 μl of Neurobasal medium and incubated with primary human neurons in MatTek plates for 18 h. Percent apoptosis/survival was determined based on the proportion of cells with DAPI-neurotubulin stain that are TUNEL positive. HIV-1ADA supernatant leads to a greater loss of neuronal viability when compared to uninfected supernatant.