Fig. 1.

Schematic overview of the expression cassette for N-terminal lipoprotein anchoring (a) and C-terminal cell wall anchoring (b) of mannanase (ManB) and chitosanase (CsnA) in L. plantarum. The vectors are derivatives of previously described plasmids [16, 17, 31]. The inserts containing the manB or csnA sequence fused with a Myc tag for protein detection. All parts are easily interchangeable using the indicated linker (L) restriction sites (SalI and EcoRI or MluI). See text for more details