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. 2016 Oct 4;15:169. doi: 10.1186/s12934-016-0570-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Western blot analysis of cell-free extracts from transformed and induced Lactobacillus cells harboring various expression vectors. (1) pEV, negative control; (2) pSIP_1261ManB (lipoprotein anchor; predicted protein size of 51 kDa); (3) pSIP_1261CsnA (lipoprotein anchor; predicted protein size of 37 kDa); (4) pSIP_ManBcwa2 (cell wall anchor; predicted protein size of 62 kDa); (5) pSIP_CsnAcwa2 (cell wall anchor; predicated protein size of 51 kDa). Lane M indicates a molecular mass marker