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. 2016 Oct 4;15:169. doi: 10.1186/s12934-016-0570-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Formation of manno-oligosaccharides (MOS) from LBG by mannanase-displaying L. plantarum cells. The picture shows HPAEC chromatograms of supernatants from the activity assay displayed in Fig. 4 (freshly harvested cells). (A) pSIP_ManBcwa2; (B) pSIP_1261ManB; (C) Standards: mannose, M1, mannobiose, M2, mannotriose, M3, mannohexaose, M6; (D) empty vector, pEV as negative control. Note that degradation of LBG galactomannan by a mannanase is expected to yield a wide variety of products (for which there are no standards), as is indeed observed in traces (A) and (B)