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. 2016 Oct 1;32(5):377–387. doi: 10.5423/PPJ.FT.08.2016.0165

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© The Korean Society of Plant Pathology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Diagram of yeast two-hybrid (Y2H) screening using Tomato yellow leaf curl virus (TYLCV) C4 bait with cDNA libraries generated from healthy and TYLCV-infected tomatoes. (A) Preparation of healthy and TYLCV-infected tomatoes. (B) Extraction of total RNA and mRNA. (C) Construction of cDNA libraries. (D) Y2H method to identify C4-interacting proteins. TYLCV C4 and tomato protein interactions were confirmed by clone survival on SD/Leu⁻/Trp⁻/His⁻ medium. AD, activation domain; BD, biding domain; UAS, upstream activating sequence; LTH, SD/Leu⁻/Trp⁻/His⁻ medium.