Table 1.
Purpose | Primer | Sequence (5′ to 3′) |
---|---|---|
Sequencing for bait (pGBKT7) and prey (pGADT7) | Matchmaker 5′ BD | TCATCGGAAGAGAGTAGTAAC |
Matchmaker 3′ BD | GAGTCACTTTAAAATTTGTAT | |
Matchmaker 5′ AD | CTATTCGATGATGAAGATAC | |
Matchmaker 3′ AD | GTGAACTTGCGGGGTTTTTC | |
Primer for first-strand cDNA synthesis | SMART III | AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCGGG |
CDS III | ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30VN | |
cDNA amplification | 5′ PCR | TTCCACCCAAGCAGTGGTATCAACGCAGAGTGG |
3′ PCR | GTATCGATGCCCACCCTCTAGAGGCCGAGGCGGCCGACA |
TYLCV, Tomato yellow leaf curl virus.