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. 2016 Oct 1;32(5):377–387. doi: 10.5423/PPJ.FT.08.2016.0165

Table 1.

Primers for sequencing of pGBKT7 and pGADT7 and amplifying cDNA inserted in pGADT7 of putative interacting clones from healthy and TYLCV C4-infected tomatoes

Purpose Primer Sequence (5′ to 3′)
Sequencing for bait (pGBKT7) and prey (pGADT7) Matchmaker 5′ BD TCATCGGAAGAGAGTAGTAAC
Matchmaker 3′ BD GAGTCACTTTAAAATTTGTAT
Matchmaker 5′ AD CTATTCGATGATGAAGATAC
Matchmaker 3′ AD GTGAACTTGCGGGGTTTTTC
Primer for first-strand cDNA synthesis SMART III AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCGGG
CDS III ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30VN
cDNA amplification 5′ PCR TTCCACCCAAGCAGTGGTATCAACGCAGAGTGG
3′ PCR GTATCGATGCCCACCCTCTAGAGGCCGAGGCGGCCGACA

TYLCV, Tomato yellow leaf curl virus.