Table 1.
List of the primers used to detect assembled sequences of five viral genomes
| Contig ID | Annotation (BLASTn) | Primer | Sequence (5′-3′) | Expected size (bp) |
|---|---|---|---|---|
| 726702 | ACLSV | 726702_For | GTCTCTTACCCAACTTTTGA | 410 |
| 726702_Rev | ATGCTGACGAACAAGCAGTC | |||
| 1012480 | AGCaV | 1012480_For | GAAGTTCAGCCCGATTGT | 510 |
| 1012480_Rev | GGTGGAAATAGCTGAATCTC | |||
| 93549 | AGCaV | 93549_For | AAGTGTTAAGTCAACTGCAG | 720 |
| 93549_Rev | CTGTATGAGACATTGATTGT | |||
| 281674 | ASGV | 281674_For | AGACTTGGGTCATTGGCAATAAT | 630 |
| 281674_Rev | TGGCGTCCTTGTAAAGCTCCGAT | |||
| 540265 | ASGV | 540265_For | TCTTGACATGGAAAAGAATG | 430 |
| 540265_Rev | ACATGCTAGAGTTTGGCCAG | |||
| 846750 | ASGV | 846750_For | AATCTCTGGG CATGTACAAT | 760 |
| 846750_Rev | GACACGTCAA AGTCGTCCCT | |||
| 34776 | ASGV | 34776_For | CACCATCAAGCTTCAGATGACGT | 403 |
| 34776_Rev | TCAACATGGGACCAAATCTGAAC | |||
| 121616 | ASPV | 121616_For | GGAATCAGATTATGAGGCAT | 680 |
| 121616_Rev | CACATATCTGAAATTGAGAT | |||
| 271566 | ASPV | 271566_For | TCACTCAACTGGAAGGATGACTT | 840 |
| 271566_Rev | GTCAAGGAAGACTAGGTTACCATGA | |||
| 273023 | ASPV | 273023_For | GTATTGGCTCCACTTCGGCTCTT | 490 |
| 273023_Rev | TCATGTCCAGGCACACTTTCAAC | |||
| 307196 | ASPV | 307196_For | AGTACACCCTTTGGGACAGCATAC | 644 |
| 307196_Rev | ACTGCTCTGTGGTGATATCACCA | |||
| 320605 | ASPV | 320605_For | GAATGTGAATTGTCAGATTTGAC | 420 |
| 320605_Rev | TATTACACATCCATTCACGG GC | |||
| 343250 | ASPV | 343250_For | GAGTCTGATTATGAGGCTTTTGAT | 456 |
| 343250_Rev | CCAGATTCCTAGTCTCTCTGGC | |||
| 417032 | ASPV | 417032_For | CAATCCGTGGTGCTATACCTTC | 493 |
| 417032_Rev | GTCTGGCAACATATTTGTGTTCA | |||
| 188422 | ASPV | 188422_For | GATGATCACAGCAAGAATAG | 710 |
| 188422_Rev | TTGATGAAAGTGTAATCGGC | |||
| 89192 | ASPV | 89192_For | GGTTCTTCTTTCATGATGAG | 700 |
| 89192_Rev | ACCACTTTAAAGTTTCTAGA | |||
| 136415 | ASPV | 136415_For | TATAGCCTTCCCAGGGGATT | 530 |
| 136415_Rev | CAGTTCTAGGTAAACCAGCC | |||
| 141873 | ASPV | 141873_For | TATTATGGGCTTGAGGCTGTGCG | 580 |
| 141873_Rev | ATACCTCATGTATGGAGCAAATC | |||
| 204809 | ASPV | 204809_For | TGGATGTGTGATAAGAGCAATCGC | 530 |
| 204809_Rev | CGAATGTACCCAGAATCATTATCA | |||
| 302849 | ASPV | 302849_For | GAAGTCCTAAAGGCTGCAACATTG | 634 |
| 302849_Rev | CCGGGAGATTTTTCGATGAA | |||
| 305081 | ASPV | 305081_For | GAA GCTGTGAGAG CTCAATGGGT | 353 |
| 305081_Rev | GCATGCACTGCATCACTCATCA | |||
| 86056 | ASPV | 86056_For | TGGGATCGTCTCAAGATCTATC | 580 |
| 86056_Rev | ATGATCAGGGTCATAAATATCCT | |||
| 281854 | ASPV | 281854_For | ACTTGGTCGTCG CGAAGTTGAC | 372 |
| 281854_Rev | TGGAAGCTCGATCAATTGATGGT | |||
| 65587 | ApLV | 65587_For | GGCTGAGAGGTATGAGTCCA | 650 |
| 65587_For | CGATCAAGTCTGGAGGGAGC | |||
| 116777 | ApLV | 116777_For | AGATCATCCTTCCAGTTGAG | 430 |
| 116777_Rev | TCTTCAATGGATTCGAGGCT |
ACLSV, Apple chlorotic leaf spot virus; AGCaV, Apple green crinkle associated virus; ASGV, Apple stem grooving virus; ASPV, Apple stem pitting virus; ApLV, Apricot latent virus.