Figure 1.

Effect of fraxetin on heme oxygenase-1 expression in HaCaT cells. (A) Chemical structure of fraxetin. (B) HaCaT cells were treated with indicated concentration of fraxetin for 24 hours and 48 hours, and cell viability was evaluated by the MTS assay. The results are presented as means ± SD (n = 3). (C, D) Cell were treated with indicated concentration of fraxetin for 6 hours. (E, F) Cell were treated with fraxetin (100 μM) for indicated time. (C, E) Expression of heme oxygenase-1 (HO-1) mRNA (ho-1) was detected by reverse transcriptase-PCR and (D, F) protein level of HO-1 was determined by Western blot analysis.