Figure 3.

Effect of fraxetin on the activation of Akt and AMP-activated protein kinase α and expression of PTEN in HaCaT cells. (A) HaCaT cells were treated with fraxetin (100 μM) for indicated periods. Cells were pretreated with (B) Akt inhibitor LY294002 (10 μM) and (C) AMP-activated protein kinase (AMPK)α inhibitor compound C (10 μM) for 1 hour prior to incubation with fraxetin (100 μM) for 1 hour. Levels of Akt and heme oxygenase-1 (HO-1) were examined by Western blot analysis. PTEN, phosphatase and tensin homolog.