Figure 4.

Roles of Akt and AMP-activated protein kinase α in fraxetin-induced nuclear factor-erythroid-2-related factor-2 activation and heme oxygenase-1 expression in HaCaT cells. Cells were pretreated with LY294002 (10 μM) (A) and compound C (10 μM) (B) for 1 hour prior to incubation with fraxetin (100 μM) for 1 hour. Expression of nuclear factor-erythroid-2-related factor-2 (Nrf2) was examined by Western blot analysis. Data are representative of three different experiments. NE, nuclear extract.