Figure 5.

Effect of fraxetin on generation of reactive oxygen species in HaCaT cells. Cells were treated with indicated concentration of fraxetin for 1 hour and then examined for the intracellular accumulation of reactive oxygen species (ROS) (A) under the fluorescence microscope using 2′-,7′-dichlorofluorescin diacetate fluorescence staining method (× 200) and (B) ELISA plate reader. The experiment was done in triplicate and the data are presented as mean ± SD. RFU, relative fluorescence unit. *P < 0.01.