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. 2016 Jul 27;5(9):1343–1350. doi: 10.1242/bio.019943

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Fluorescence microscopy of LYVE-1 surface distribution in HDLECs using conjugated secondary antibody. Confocal images of LYVE-1 surface distribution in HDLEC visualised with Oregon Green^® 488 conjugated secondary antibody (as in Fig. 1E) added after fixation with 1% (w/v) (left panels) and 4% (w/v) PFA (right panels) in (A) the absence and (B) presence of 0.2% (w/v) GA. Scale bars: 5 µm. (C) Analysis of LYVE-1 cluster sizes as imaged by STED microscopy following 1% PFA and GA fixation protocols: cluster size (top) and width (bottom), depicting a reduction in size with increasing in GA concentration. Error bars: mean±s.e.m. Average cluster size and surface area from 10 cells. ****P<0.0001; 1-way ANOVA with Dunnett's multiple comparisons.