Fig. 5.

FRAP data of LYVE-1 in permeabilised HDLECs (labelled with primary antibody mAb fluorescent conjugate). No mobility is observed for cells fixed with (A) 1% PFA or (B) 1% PFA+0.2% GA and permeabilised with 0.1% Triton-X and 0.2% Saponin, or cells permeabilised with (C) ice-cold methanol (grey curve original ‘fluorescence recovery’ data as average over at least 5 measurements at different sample positions and with error bars depicting the standard deviation of the mean; and blue curve ‘bleaching control’ data taken without photobleaching pulse depicting photobleaching during observation).