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. 2016 Oct 5;11(10):e0162636. doi: 10.1371/journal.pone.0162636

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© 2016 Biswas, Longmore

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — SNAIL1-deleted cardiac fibroblasts have decreased αSMA, LoxL2 & Periostin expression. (A) αSMA immunofluorescence of control (CTL) and SNAIL1-deleted (+ Cre) cardiac fibroblasts. (B) Quantification of results in (A). 30 fields were counted in each group. Shown is a representative result of one of 3 independent experiments. (C) Gel contraction assay of control (CTL) and SNAIL1^-/- (+ Cre) cardiac fibroblasts. (D) Quantification of (C). Shown is pooled data from 3 independent experiments, done in triplicates. (E-J) Relative fibrogenic gene mRNA expression in SNAIL1^-/- cardiac fibroblasts following TGFβ stimulation: IL6 (E), TGFβ (F), CTGF (G), LoxL2 (H), Periostin (I and J). Representative example of 3 separate experiments. (K) Western blot analysis with periostin antibody of extracts from control (CTL) and SNAIL1^-/- cardiac fibroblasts. Representative example of one of three separate experiments.