Figure 2. Enhancement of EPSC_NR2AR and EPSC_NR2BR by CCL2.

Panels A and B illustrate the time course and amplitude (% of baseline) of the EPSC_NR2AR and EPSC_NR2BR recorded, in the presence of ifenprodil (Panel A, to block NR2BR, n=10) and R-CPP (Panel B, to block NR2AR, n=12), from two different neuronal cells in the CA1 region of two different slices taken from the same animal in response to constant current stimulation of Schaffer-collateral fibers (80μA, 40μS, 0.05Hz). Each data point plots the average of three consecutive EPSCs. Note that bath application of CCL2, as indicated a horizontal bar, increased the EPSC_NR2AR (A) and EPSC_NR2BR (B). Above each time course graph are representative individual EPSC_NR2AR (A) and EPSC_NR2BR (B) taken from different time points as marked by numbers 1, 2, 3, and 4, respectively. The CCL2-induced increase of EPSC_NR2AR or EPSC_NR2BR was almost completely blocked by a specific NA2AR antagonist R-CPP (A) or a specific NR2BR blocker ifenprodil (B). Panel C is a bar graph exhibiting the average amplitudes of EPSC_NR2AR or EPSC_NR2BR before (control), during (CCL2) and post (Wash) bath application of CCL2. CCL2 enhanced both EPSC_NR2AR or EPSC_NR2BR. All experiments were carried out in the presence of CNQX (10μM) in the perfusate and the cells were voltage clamped at −50mV. *p<0.05.