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. 2016 Oct 5;12(10):e1005927. doi: 10.1371/journal.ppat.1005927

Fig 2. HPG incorporation has no effect on the accumulation of candidate viral proteins nor on overall virus yield.

Fig 2

(A) Vero cells were pulse-labeled with 0.5 mM HPG for 30 min at 2, 8 or 16 hr p.i. and lysates were collected at the termination of each pulse. Control cultures were incubated as described for Fig 1 (Con and Met). Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes. Total steady-state levels of early and late viral proteins, ICP8 and VP5 (red) respectively, were simultaneously detected and γ-tubulin (green) was used as loading control. (B) Diagram illustrating the protocol for experiments in (C) and (D). Mock or infected Vero cells were pulse-labeled with 0.5 mM HPG for 30 min at 2, 4, 6, 8, 16 or 20 hr p.i. and chased in normal methionine-containing medium after removal of HPG, harvested at 20.5 hr post infection and analysed for total VP5 and ICP8 accumulation (C) and for total infectious viral yields (D).