Fig 3. Imaging newly synthesised proteins by HPG incorporation and click chemistry.

Uninfected Vero cells were pulse-labeled using 0.5 mM HPG for 30 min, fixed and subjected to click reaction using the Alexa Fluor 488-azide capture agent. Control experiments were performed either in the absence of HPG or using 100 μg/ml of CHX added prior to and during the pulse. Images were recorded as described in materials and methods.