Skip to main content
. 2016 Oct 5;11(10):e0164097. doi: 10.1371/journal.pone.0164097

Fig 2. Induction of pathogen-inducible promoter 4XW2 in response to Pep25 and X. campestris pv. vesicatoria.

Fig 2

The 4XW2-GFP-GUS was transiently transformed into TH3 tomato protoplasts using a modified PEG method. GFP fluorescence was monitored 12–16 h after water (A and B), pep25 (C and D) and X. campestris pv. vesicatoria treatment (E and F). (G) Number of protoplasts showing GFP fluorescence is given in percent. (H) Quantification of GFP fluorescence intensity was done using the Image J software (http://imagej.nih.gov/ij/). Scale bar: 20 μm. Asterisks indicate significant different in comparison to the corresponding control treatment, *P<0.05, **P<0.01, ***P<001.