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. 2016 Mar 16;3:16004. doi: 10.1038/mtm.2016.4

Table 2. Estimated recovery from 1 l of FV-hCD18 vector after each step of purification.

Step Volume of vector (ml) Processing time % of step recovery (average ± SEM)a n
Heparin column 333.3 5 hours 69 ± 2.7 5
TFF 11.9 45 minutes 89 ± 5.8 5
0.2 m filter 11.9 15 minutes 84 ± 4.5 5
Ultracentrifugation 0.2 2 hours 48 ± 6.3 5
Net recovery 0.2 8 hoursb 19 ± 3.1 5
a

Data represent mean and standard error of mean (SEM) of five independent experiments.

b

Total processing time.