(a) Dry preference assessed on ~67% to ~96% gradient. Asterisks denote statistically different responses from wild type (**p<0.01; Steel with control). wild type, n = 16 assays. nan mutant (nan36a), n = 9. wtrw mutant (wtrw2), n = 9. (b–e) Moisture-responsive fluorescence changes of IR40a neurons recorded and quantified as described in Figure 5. Genotypes: control: n = 5 animals (Ir40a-Gal4,UAS-GCaMP6m/+). nan mutant (Ir40a-Gal4,UAS-GCaMP6m/+;nan36a), n = 5. wtrw mutant (Ir40a-Gal4,UAS-GCaMP6m/+ ;wtrw2), n = 7. (All P>0.4 versus control, Steel with control).
DOI:
http://dx.doi.org/10.7554/eLife.17879.008