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. 2016 Oct;26(10):1355–1362. doi: 10.1101/gr.205955.116

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© 2016 Chen et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Identification of Type II essential genes and their rescuing partners. (A) A flowchart of the experimental procedure. (B) The colony size of each of the double gene deletion segregants. Cells diluted in gradient were spread on a YPD plate and incubated for 48 h, except for the ΔMIM1 + ΔSIT4, which was incubated for 72 h. Each panel shows a Type II essential gene and its rescuing partners. (C) The fitness of each double gene deletion segregant relative to the wild type in YPD.