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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Steroids. 2016 May 15;114:7–15. doi: 10.1016/j.steroids.2016.05.003

Figure 4.

Figure 4

Chromatin accessibility mediates cell type-specific actions of GR. ChIP-Seq tracks for GR, H3K4me3, H3K4me1 and H3K27ac in liver and inflammatory macrophages. (A) For Per1, activated by GR in both tissues, the GR peaks lie in close proximity to H3K4 monomethylated chromatin – a histone mark associated with enhancers. This enhancer is active in hepatocytes (overlapping H3K27ac) or poised in macrophages (missing H3K27ac). (B) In hepatocytes but not macrophages, GR binds close to the Pck1 promoter. The surrounding chromatin harbors the H3K4me1/H3K27ac double mark for active enhancers as well as a H3K4 trimethyation mark, indicating the proximity to the Pck1 promoter. Macrophage chromatin at this locus contains neither enhancer/promoter-specific histone marks nor a GBS. (C) The Il6 enhancer contains a GBS in macrophages, which overlaps with H3K4me1/H3K27ac chromatin, indicating an active enhancer. In hepatocytes this chromatin is not accessible for GR binding and harbors none of these marks.