Skip to main content
. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Bioorg Med Chem. 2016 Apr 26;24(20):4779–4786. doi: 10.1016/j.bmc.2016.04.050

Figure 5.

Figure 5

Thermally-induced strand cleavage of w794 DNA following DNA alkylation; DNA–agent incubation at 23 °C for 24 h, removal of unbound agent by EtOH precipitation, and 30 min of thermolysis (100 °C) followed by 8% denaturing PAGE and autoradiography. Lane 1, control DNA; lanes 2–5, Sanger G, C, A, and T sequencing reactions; lanes 6–8, (–)-15 (natural enantiomer at 1 × 10−5, 1 × 10−6, and 1 × 10−7 M−1).