Table 1.

Approximation of the degree of undersampling in transduced polyclonal hPSC

Total cell # harvested		% of each lineage from FACS	Cell # yield expecteda	Cell # yield obtained from FACS	gDNA quant. (uc378/ul)b	vol of gDNA eluted (ul)	total cell equivalent after gDNA extractionc	vol/ nrLAM PCR (ul)	# nrLAM PCR reactions	total cell equivalent for nrLAM PCR libraries inputd	% recovery after FACSe	% recovery after DNA isolationf	% recovery after library prepg
3.10E+07	H	22.41%	6.95e6	6.44e6	27600	100	1.38e6	1	10	1.38e5	92.64%	21.44%	2.14%
	E	2.78%	8.60e5	8.57e5	11280	50	2.82e5	1	10	5.64e4	99.67%	32.89%	6.58%
	M	0.98%	3.04e5	2.26e4	2580	50	6.45e4	1.5	10	1.94e4	74.10%	28.60%	8.58%

^aCell yield expected is calculated as [Total cell# harvested from OP9 week2] æ [% of each lineage from FACS]

^bAbsolute gDNA quantification is performed by droplet digital PCR using uc378 as a diploid reference target locus

^cTotal cell equivalent after gDNA extraction is calculated as ([gDNA quantification (uc378/ul)] æ [volume of gDNA eluted (ul)])¼2, assuming that each diploid cell contains two copies of uc378

^dTotal cell equivalent for nrLAM PCR libraries input is calculated as ([volume/nrLAM PCR] æ [#nrLAM PCR] æ [total cell equivalent after gDNA extraction])¼[volume of gDNA eluted (ul)]

^e%recovery after FACS is calculated as ([Cell yield obtain from FACS sorter]¼[Cell yield expected]) æ 100

^f%recovery after DNA isolation is calculated as ([total cell equivalent after gDNA extraction]¼[Cell yield obtain from FACS sorter]) æ 100

^g%recovery after library prep is calculated as ([total cell equivalent for nrLAM PCR libraries input]¼[Cell yield obtain from FACS sorter]) æ 100