Fig. 1.

Effects of C₆₀ derivatives on T cell responses. a C₆₀-P or C₆₀(OH)₃₆ was added to responder splenocytes 30 min before the addition of stimulator splenocytes. After incubation of the cells for 4 days, the amount of IL-2 in the supernatants was measured by ELISA. b One week after the third immunization with OVA and alum, mice were euthanized and single-cell suspensions of splenocytes were prepared. C₆₀-P or C₆₀(OH)₃₆ was added to the wells 30 min before the addition of OVA (100 μg/mL). After incubation of the cells for 3 days, the amount of IL-4 in the supernatants was measured by ELISA. c Purified CD4⁺ T cells were added to anti-CD3-coated plates. Each C₆₀ derivative or N-acetylcysteine (NAC) was added to the wells 30 min before the addition of anti-CD28. After incubation of the cells for 3 days, the amount of IL-2 in the supernatants was determined by ELISA. Data are means ± SDs for three to six independent cultures (n = 3 to 6). *P < 0.05 vs. control group