Fig. 7.

Expression levels of seven genes (cla4, spa2, ras2, ras1, sepa, cdc42, and pxl) involved in branching in RUT C30 and DES-15. (a–c) Transcription abundances of cla4, ras2, ras1, spa2, rhoA, cdc42, and racA. Mycelia were collected at 0 (before batch), 24, and 72 h. The gpd1 gene was used for normalization, and the values were calculated by 2^−ΔΔCt methods. Experiments were performed in three independent replicates. Error bars represent standard deviations. Asterisks were indicated significant differences at P < 0.05 according to Student’s t tests