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. 2016 Oct 6;10:235. doi: 10.3389/fncel.2016.00235

FIGURE 4.

FIGURE 4

(A) Putative binding sites of miR-23b within TUSC7 mRNA, and the sequences of wild-type and mutant-type vectors. (B) MiR-23b was highly expressed in human glioma tissues and cell lines. (C) Compared with control groups, agomir-23b increased the expression of miR-23b in U251 and U87 cells, antagomir-23b inhibited miR-23b expression. (D) The relative luciferase activities were inhibited in the HEK-293 cells co-transfected with wild-type vector and p agomir-23b, and not with the mutant-type vector. Firefly luciferase activity was normalized to Renilla luciferase. (E) Detection of TUSC7 mRNA using qRT-PCR in the sample pulled down by biotinylated miR-23b. (F) Detection of miR-23b mRNA using qRT-PCR in the sample pulled down by biotinylated TUSC7. (G) MiR-23b expression was down-regulated significantly by TUSC7 over-expression in U251 and U87 cell lines. (H,I) Association of TUSC7 and miR-23b with Ago2 in U251 and U87 cells. Cellular lysates from U251 and U87 cells were used for RIP with antibody against Ago2. TUSC7 and miR-23b expression levels were detected using qRT-PCR. ∗P < 0.05.