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. 2016 Oct 6;7:409. doi: 10.3389/fimmu.2016.00409

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Copyright © 2016 Yu, Long, Li, Liang, Liao, Yuan and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-9 expressions were increased in myocardium of VMC mice. (A) The results of immunohistochemistry (magnification 400×) in the heart tissue showed that IL-9 distributed in the lesions with inflammation on days 5 and 7. (B) CD45⁺ cells were gated. And the isotype control of IL-9 was showed. (C) The representative pictures for IL-9-secreting leukocyte (CD45⁺IL-9⁺) levels in different groups. (D) The results of statistical analysis for IL-9-secreting leukocyte levels by flow cytometry in different groups. (E) The CD45⁺IL-9⁺ cells were gated and further analyzed for CD11b, Gr-1, CD49b, CD4, and CD8 expressions to detect the cellular source of IL-9. **P < 0.01 vs. control group; ^##P < 0.01 vs. IL-9KO group. Values are means ± SEM. Ten mice were euthanized in each group separately on days 5 and 7.

IL-9 expressions were increased in myocardium of VMC mice. (A) The results of immunohistochemistry (magnification 400×) in the heart tissue showed that IL-9 distributed in the lesions with inflammation on days 5 and 7. (B) CD45⁺ cells were gated. And the isotype control of IL-9 was showed. (C) The representative pictures for IL-9-secreting leukocyte (CD45⁺IL-9⁺) levels in different groups. (D) The results of statistical analysis for IL-9-secreting leukocyte levels by flow cytometry in different groups. (E) The CD45⁺IL-9⁺ cells were gated and further analyzed for CD11b, Gr-1, CD49b, CD4, and CD8 expressions to detect the cellular source of IL-9. **P < 0.01 vs. control group; ^##P < 0.01 vs. IL-9KO group. Values are means ± SEM. Ten mice were euthanized in each group separately on days 5 and 7.