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Proteolytic processing of pro-Cry1Ac to active toxin by midgut bacterial proteases. Proteases (30 μg) from midgut bacteria were incubated with pro-Cry1Ac (10 μg) for 1 h. Active Cry1Ac (band at 65 kDa) was detected on (A) SDS-PAGE [molecular markers (MMs), lane 1- pro-Cry1Ac, lane 2- active Cry1Ac, lane 3 – IVS1, lane 4 – IVS2 and lane 5 – IVS3] and (B) Western blot (lane 1- IVS1, lane 2- IVS2, and lane 3- IVS3).
