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. 2016 Oct 6;7:1567. doi: 10.3389/fmicb.2016.01567

FIGURE 7.

FIGURE 7

Cry1Ac toxicity in antibiotic cocktail fed H. armigera larvae. Larvae were reared until the fourth-instar on sterile artificial diet amended with 100 μl of antibiotic cocktail (Stock: 1 mg/ml of each antibiotic) per gram of diet. The antibiotic pre-treated early fourth-instar larvae were transferred to artificial diet treated with gut bacterial culture and pro-Cry1Ac (15 μg/ml of diet) and reared for 5 days. Data was recorded for Cry1Ac toxin mortality in larvae reared on artificial diet, antibiotic cocktail treated diet and gut bacteria treated diet. Equal amount of culture from IVS1, IVS2, and IVS3 isolates was added to the artificial diet. There were three replications for each treatment and 10 larvae in each replication in a CRD. Each value represents the average of three experiments.