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Quantification of active toxin produced from pro-Cry1Ac by gut bacterial proteases.
| Isolate | Cry1Ac (μg)/mg protein |
|---|---|
| IVS1 | 0.20 ± 0.03 |
| IVS2 | 0.12 ± 0.01 |
| IVS3 | 0.15 ± 0.02 |
| IVS4 | – |
| IVS5 | – |
| IVS6 | – |
| IVS7 | – |
Bacterial proteases (50 μg) incubated with pro-Cry1Ac for 1 h. Sample (100 μl) was added to ELISA plate wells and the reaction was carried out as described in M&Ms. The values were represented as mean ± SE.
