Table 4.

Effect of proteases inhibitors on proteases from H. armigera gut bacteria.

Inhibitor	Residual activity (%)
	IVS1		IVS2		IVS3
	Total protease	Chymotrypsin	Total protease	Chymotrypsin	Total protease	Chymotrypsin
Control	100 ± 0.0 h	100 ± 0.0 h	100 ± 0.0 f	100 ± 0.0 f	100 ± 0.0 f	100 ± 0.0 g
TPCK	54 ± 1.5 c	81 ± 2.7 e	61 ± 0.8 d	75 ± 2.0 c	65 ± 2.0 c	80 ± 0.8 d
Chymostatin	59 ± 1.5 d	11 ± 1.5 a	56 ± 1.5 c	6 ± 0.8 a	65 ± 2.5 c	6 ± 0.8 a
E64	69 ± 1.9 ef	74 ± 0.8 c	56 ± 2.3 c	100 ± 0.0 f	73 ± 1.5 d	80 ± 2.3 d
Leupeptin	73 ± 3.4 g	95 ± 2.3 g	56 ± 0.8 c	98 ± 0.8 e	65 ± 2.0 c	92 ± 1.5 f
Aprotonin	68 ± 0.8 e	83 ± 2.0 ef	67 ± 1.5 e	100 ± 0.0 f	75 ± 2.3 e	85 ± 1.5 e
EDTA	42 ± 2.8 b	77 ± 1.5 cd	39 ± 2.0 b	93 ± 1.5 d	45 ± 1.5 b	76 ± 2.0 c
PMSF	24 ± 1.9 a	15 ± 0.8 b	31 ± 0.8 a	9 ± 0.8 b	26 ± 0.8 a	10 ± 0.8 b

The protease activity was measured in the presence of inhibitors listed. Activity in the absence of inhibitors was taken as 100% (control). Values are significant at P < 0.05. Values with different letters in the columns are significantly different from each other. Each value represents the average of three experiments and represented as mean ± SE.