Table 5.
LT50 (days) values of B. bassiana, M. anisopliae and I. fumosorosea against larvae of M. domestica by immersion and bait methods.
| Fungi | Isolate | Immersion method |
Bait method |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Concentration (spores/mL) | %Mortality | LT50 | FDa | Slope | χ2 | %Mortality | LT50 | FDa | Slope | χ2 | ||
| B. bassiana | Bb-01 | 3 × 108 | 86.00 ± 1.15a | 5.24 | 4.63–5.58 | 6.52 ± 2.09 | 18.09 | 79.00 ± 5.26a | 5.50 | 4.62–6.53 | 5.87 ± 0.93 | 17.33 |
| 2 × 108 | 75.00 ± 3.00ab | 5.61 | 5.31–5.92 | 5.48 ± 0.48 | 15.96 | 65.00 ± 3.00ab | 6.10 | 5.73–6.49 | 5.38 ± 0.51 | 8.94 | ||
| 1 × 108 | 68.00 ± 3.65ab | 5.91 | 5.13–6.81 | 5.59 ± 0.81 | 12.49 | 57.00 ± 1.00b | 6.47 | 6.04–6.93 | 5.28 ± 0.54 | 11.06 | ||
| 1 × 107 | 49.00 ± 1.45c | – | – | – | – | 42.00 ± 3.21c | – | – | – | – | ||
| 1 × 106 | 36.00 ± 2.45cd | – | – | – | – | 35.21 ± 2.00 cd | – | – | – | – | ||
| Bb-08 | 3 × 108 | 72.00 ± 4.32ab | 5.88 | 5.15–6.73 | 6.37 ± 3.77 | 21.16 | 67.00 ± 2.52a | 6.36 | 5.98–6.92 | 5.64 ± 1.64 | 12.34 | |
| 2 × 108 | 61.00 ± 5.26ab | 6.28 | 5.17–7.55 | 5.46 ± 0.93 | 15.03 | 58.00 ± 3.46b | 6.59 | 6.09–7.12 | 4.73 ± 0.49 | 2.96 | ||
| 1 × 108 | 48.00 ± 2.11c | – | – | – | – | 55.00 ± 1.00b | 6.60 | 6.13–7.10 | 5.15 ± 0.53 | 8.66 | ||
| 1 × 107 | 38.00 ± 1.45cd | – | – | – | – | 37.00 ± 3.45c | – | – | – | – | ||
| 1 × 106 | 30.00 ± 2.11d | – | – | – | – | 27.00 ± 1.56 cd | – | – | – | – | ||
| Bb-10 | 3 × 108 | 68.00 ± 4.32ab | 6.04 | 5.67–6.44 | 5.10 ± 0.48 | 7.26 | 63.00 ± 4.43ab | 6.26 | 5.84–6.71 | 4.95 ± 0.49 | 6.01 | |
| 2 × 108 | 54.00 ± 1.15b | 6.80 | 6.22–7.44 | 4.32 ± 0.46 | 0.85 | 51.00 ± 2.52bc | 7.06 | 6.41–7.79 | 4.25 ± 0.47 | 0.68 | ||
| 1 × 108 | 47.00 ± 2.34bc | – | – | – | – | 44.00 ± 3.54bc | – | – | – | – | ||
| 1 × 107 | 36.00 ± 3.56cd | – | – | – | – | 32.00 ± 1.56bc | – | – | – | – | ||
| 1 × 106 | 28.00 ± 3.67d | – | – | – | – | 25.00 ± 3.21cde | – | – | – | – | ||
| M. anisopliae | Ma-2.3 | 3 × 108 | 78.00 ± 2.58ab | 5.50 | 5.19–5.81 | 5.08 ± 0.44 | 7.54 | 67.00 ± 1.91ab | 6.28 | 4.83–8.17 | 5.48 ± 1.10 | 7.76 |
| 2 × 108 | 63.00 ± 4.12b | 6.27 | 5.97–6.77 | 4.29 ± 0.42 | 2.95 | 56.00 ± 2.83b | 6.65 | 6.15–7.19 | 4.89 ± 0.51 | 3.79 | ||
| 1 × 108 | 53.00 ± 3.00bc | 7.04 | 6.38–7.79 | 4.13 ± 0.45 | 1.59 | 46.00 ± 3.45bc | – | – | – | – | ||
| 1 × 107 | 40.00 ± 2.45bc | – | – | – | – | 38.00 ± 4.11c | – | – | – | – | ||
| 1 × 106 | 34.00 ± 2.11cd | – | – | – | – | 31.00 ± 3.54c | – | – | – | – | ||
| Ma-4.1 | 3 × 108 | 72.00 ± 3.65ab | 5.66 | 5.33–6.04 | 4.74 ± 0.43 | 3.50 | 69.00 ± 4.12ab | 5.88 | 5.56–6.22 | 5.53 ± 0.51 | 10.35 | |
| 2 × 108 | 61.00 ± 3.42b | 6.42 | 5.90–6.99 | 4.15 ± 0.42 | 2.48 | 60.00 ± 3.65b | 6.48 | 5.99–6.99 | 4.70 ± 0.48 | 1.83 | ||
| 1 × 108 | 52.00 ± 2.31bc | 7.05 | 6.42–7.75 | 4.44 ± 0.49 | 1.46 | 52.00 ± 2.31bc | 7.00 | 6.42–7.62 | 4.88 ± 0.54 | 5.38 | ||
| 1 × 107 | 45.00 ± 3.11c | – | – | – | – | 42.00 ± 3.21bc | – | – | – | – | ||
| 1 × 106 | 31.00 ± 2.45cd | – | – | – | – | 34.00 ± 3.67c | – | – | – | – | ||
| Ma-11.1 | 3 × 108 | 61.00 ± 3.42b | 6.48 | 6.03–6.96 | 5.07 ± 0.52 | 6.96 | 59.00 ± 4.73b | 6.62 | 6.13–7.15 | 4.92 ± 0.51 | 5.26 | |
| 2 × 108 | 54.00 ± 3.83bc | 6.94 | 6.26–7.69 | 3.81 ± 0.41 | 1.41 | 51.00 ± 4.43bc | 7.09 | 6.44–7.81 | 4.38 ± 0.48 | 1.08 | ||
| 1 × 108 | 42.00 ± 3.21c | – | – | – | – | 39.00 ± 2.00bc | – | – | – | – | ||
| 1 × 107 | 31.00 ± 3.27cd | – | – | – | – | 28.00 ± 3.11d | – | – | – | – | ||
| 1 × 106 | 23.00 ± 2.45d | – | – | – | – | 15.00 ± 1.56de | – | – | – | – | ||
| I. fumosorosea | If-02 | 3 × 108 | 56.00 ± 2.31bc | 6.88 | 5.77–7.89 | 5.65 ± 0.42 | 9.47 | 52.00 ± 4.32bc | 7.49 | 6.71–8.36 | 4.24 ± 0.50 | 6.08 |
| 2 × 108 | 39.00 ± 2.45cd | – | – | – | – | 45.00 ± 2.54bc | – | – | – | – | ||
| 1 × 108 | 31.00 ± 1.45cd | – | – | – | – | 36.00 ± 1.34cd | – | – | – | – | ||
| 1 × 107 | 27.00 ± 1.54d | – | – | – | – | 25.00 ± 2.32de | – | – | – | – | ||
| 1 × 106 | 18.00 ± 2.67de | – | – | – | – | 20.00 ± 1.32d | – | – | – | – | ||
| If-2.3 | 3 × 108 | 68.00 ± 4.32ab | 6.03 | 4.99–7.30 | 5.57 ± 0.95 | 6.31 | 57.00 ± 6.19bc | 6.62 | 5.44–8.05 | 5.27 ± 0.90 | 13.52 | |
| 2 × 108 | 57.00 ± 2.52bc | 6.64 | 6.15–7.16 | 5.01 ± 0.52 | 5.91 | 47.00 ± 4.32bc | – | – | – | – | ||
| 1 × 108 | 47.00 ± 3.45c | – | – | – | – | 40.00 ± 1.42c | – | – | – | – | ||
| 1 × 107 | 35.00 ± 3.11cd | – | – | – | – | 29.00 ± 3.11cd | – | – | – | – | ||
| 1 × 106 | 28.00 ± 4.21d | – | – | – | – | 27.00 ± 2.67cd | – | – | – | – | ||
| If-03 | 3 × 108 | 61.00 ± 3.42b | 6.57 | 5.59–7.43 | 5.60 ± 1.73 | 14.32 | 53.00 ± 3.00bc | 6.88 | 6.33–7.48 | 4.89 ± 0.53 | 5.04 | |
| 2 × 108 | 53.00 ± 1.00bc | 7.02 | 5.01–9.43 | 5.31 ± 1.45 | 9.62 | 45.00 ± 2.54bc | – | – | – | – | ||
| 1 × 108 | 46.00 ± 2.87c | – | – | – | – | 36.00 ± 3.11cd | – | – | – | – | ||
| 1 × 107 | 37.00 ± 2.00cd | – | – | – | – | 30.00 ± 1.76cd | – | – | – | – | ||
| 1 × 106 | 27.00 ± 3.21d | – | – | – | – | 17.00 ± 2.15de | – | – | – | – | ||
| F-value | 15.3 | 25.1 | ||||||||||
| p-value | <0.0001 | <0.0001 | ||||||||||
| LSD-value | 18.35 | 18.17 | ||||||||||
a
Fiducial limit.
Means followed by the same letters in columns are not significantly different at the 5% level.