Table 1.
Primers used for the detection of canine respiratory viruses via PCR in dog samples from shelters in the Rio Grande do Sul state.
| Virus | Amplified gene | Primers (5′–3′) | Amplicon length | Reference |
|---|---|---|---|---|
| CDV | Nucleoprotein | TTCTGAGGCAGATGAGTTCTTC CTTGGATGCTATTTCTGACACT |
829 pb | 19 |
| CPIV | Nucleoprotein | GAGGCTCGACGAATAATC GTTCGGCTTGAGTTAGACC |
532 pb | This reference |
| CAdV-2 | E3 | CGCGCTGAACATTACTACCTTGTC CCTAGAGCACTTCGTGTCCGCTT |
1030 pb | 20 |
| CaHV-1 | Glycoprotein B | CCTAAACCTACTTCGGATGA GGCTTTAAATGAACTTCTCTGG |
450 pb | 21 |
CDV, canine distemper virus; CPIV, canine parainfluenza virus; CAdV-2, canine adenovirus type 2 and CaHV-1, canid herpesvirus 1.