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Confocal fluorescence microscopy images of GUVs. The red channel shows a rhodamine-labeled membrane and the green channel shows labeled unactivated Vn. (A) DOPC/PIP2 (9:1). (B) DPPC/DOPC/Chol (1:2:1). (C) DOPC. The lipid/protein ratio is 3000:1. The highest protein concentration for which no binding was observed was 2 μM (lipid/protein ratio 70:1). To see this figure in color, go online.
