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. 2016 Oct 4;111(7):1444–1453. doi: 10.1016/j.bpj.2016.08.019

Figure 6.

Figure 6

AFM images of PIP2 harboring Vn coupled with confocal fluorescence microscopy images. (A) PIP2 clusters that formed in DOPC/10%PIP2 membranes in the presence of divalent cations (heights ≈ 4 nm). (B) Addition of 40 nM Vac, showing PIP2 clusters along with Vac (heights ≈ 7 nm). (C) Fluorescence imaging coupled with the AFM image (B) depicting Lys-labeled Vn. (D) Three-dimensional representation of PIP2 clusters (light blue) harboring Vac molecules (pink). The lipid/protein ratio is 6000:1. To see this figure in color, go online.