Figure 2.

Subclustering of Epidermal Cell Populations

(A–D) Subclustering (second-level clustering) of epidermal cells from the IFE basal (A), upper HF (B), outer bulge (C), and inner bulge (D) compartments. Upper panel: projection of subpopulations onto the t-SNE map of the full dataset introduced in Figure 1D. Lower panel: barplots showing the expression of marker genes per subpopulation. Each bar represents a single cell, and the black line indicates the average expression over each subpopulation.

(E) Selection of immuno- and single-molecule FISH (symbols italics) stainings to visualize subpopulation localization within the tissue. Arrowheads highlight the position of the populations: IFE BI (filled arrowhead)/BII (empty arrowhead); uHF I (filled arrowhead)/II (empty arrowhead); OB III (filled arrowhead; dashed line marks lower end of KRT15 gap). HS, hair shaft. SG, sebaceous gland. CH, club hair. Scale bars, 10 μm. See also Figure S3L.

(F) Identity and marker genes of cell populations defined during second-level clustering.

(G) Summary of the approximate location of each defined subpopulation in the IFE, SG, and HF.