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. 2016 Oct 4;111(7):1409–1417. doi: 10.1016/j.bpj.2016.08.033

Figure 5.

Figure 5

(A and B) Plot of the observed force for Ton-box extraction from the luminal domain in (A) Apo-sym (green) and (B) Apo-OM (orange) systems, with the corresponding standard error shown in gray. Each curve is an average of three time-averaged, constant-velocity SMD runs of 1 Å/ns. The Apo-OM curve is the run average of one Apo2-OM and two Apo1-OM SMD runs. The peak force for Apo-sym occurs around 11 ns and corresponds to unbinding of Arg14 from the hydrogen-bond network, as shown in Fig. 4. The peak force in the Apo-OM systems occurs around 6 ns and corresponds to the hydrophobic interaction of Val10 to Ala12 with the luminal domain. Independent SMD run results can be found in Fig. S11. To see this figure in color, go online.